Intracellular proteoglycans

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Heparan sulfate proteoglycans are concentrated on the sinusoidal plasmalemmal domain and in intracellular organelles of hepatocytes

The distribution of cell surface heparan sulfate proteoglycans (HSPGs) was determined in rat liver by immunocytochemistry. A polyclonal antibody was raised against HSPGs purified from rat liver microsomes which specifically immunoprecipitated liver membrane HSPGs. It was shown to recognize both the heparin-releasable and membrane-intercalated form of membrane HSPGs and to recognize determinants...

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Altered synthesis and intracellular transport of proteoglycans by cyst-derived cells from human polycystic kidneys.

Employing in vitro pulse-chase techniques, we investigated the de novo synthesis and the kinetics of intracellular transport and extracellular matrix incorporation of proteoglycans (PG) by normal human renal epithelial cell and by epithelial cells isolated from cysts of autosomal dominant kidneys (ADPKD). Cell monolayers were pulsed either with (3H)leucine for 15 min and chased for seven interv...

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Cartilage proteoglycans.

The predominant proteoglycan present in cartilage is the large chondroitin sulfate proteoglycan 'aggrecan'. Following its secretion, aggrecan self-assembles into a supramolecular structure with as many as 50 monomers bound to a filament of hyaluronan. Aggrecan serves a direct, primary role providing the osmotic resistance necessary for cartilage to resist compressive loads. Other proteoglycans ...

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Proteoglycans and pathophysiology.

VENTILATION-INDUCED LUNG INJURY (VILI) is an important clinical entity whose pathophysiology is incompletely understood. The article by Moriondo and coworkers (14) in the Journal of Applied Physiology offers new insights into potential mechanistic pathways. These authors report that one of the earliest responses to mechanical ventilation in healthy rats is evidence of proteoglycan (PG) fragment...

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Proteoglycans in stem cells.

The remarkable promise of pluripotent and multipotent stem cells (SCs) imparts tremendous optimism for advancement of regenerative medicine, developmental biology, and drug discovery. Perhaps the greatest challenge is to finely direct, control, and command their differentiation. As those processes are managed on many levels, including genomic, transcriptomic, and epigenomic, examination of all ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2004

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20031230